Selectable markers, e.g., antibiotic resistance, for conjugation experiments are not always effective for slow-growing plant growth promoting bacteria such as Burkholderia. We used PCAT medium containing Congo Red for selecting Burkholderia transconjugants. This method allows for the reliable selection of transconjugants of these novel plant growth-promoting bacteria.
Mohsin Tariq, Michelle R. Lum, Allan W. Chong, Anjana B. Amirapu, Sohail Hameed, Ann M. Hirsch, "A reliable method for the selection and confirmation of transconjugants of plant growth-promoting bacteria especially plant-associated Burkholderia spp." Journal of Microbiological Methods, Volume 117, 2015, Pages 49-53, ISSN 0167-7012, https://doi.org/10.1016/j.mimet.2015.07.008.