G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila

Authors

Cory J. Evans, University of California, Los AngelesFollow
John M. Olson, University of California, Los Angeles
Kathy T. Ngo, University of California, Los Angeles
Eunha Kim, University of California, Los Angeles
Noemi E. Lee, University of California, Los Angeles
Edward Kuoy, University of California, Los Angeles
Alexander N. Patananan, University of California, Los Angeles
Daniel Sitz, University of California, Los Angeles
Phuong Thao Tran, University of California, Los Angeles
Minh Tu Do, University of California, Los Angeles
Kevin Yackle, University of California, Los Angeles
Albert Cespedes, University of California, Los Angeles
Volker Hartenstein, University of California, Los Angeles
Gerald B. Call, University of California, Los Angeles
Utpal Banerjee, University of California, Los Angeles
Miriam Beyder, University of California, Los Angeles
Kush V. Bhatt, University of California, Los Angeles
Chinmay Bhoot, University of California, Los Angeles
Aaron W. Bradshaw, University of California, Los Angeles
Tierney G. Brannigan, University of California, Los Angeles
Boyu Cao, University of California, Los Angeles
Yancey Y. Cashell, University of California, Los Angeles
Timothy Chai, University of California, Los Angeles
Alex W. Chan, University of California, Los Angeles
Carissa Chan, University of California, Los Angeles
Inho Chang, University of California, Los Angeles
Jonathan Chang, University of California, Los Angeles
Michael T. Chang, University of California, Los Angeles
Patrick W. Chang, University of California, Los Angeles
Stephen Chang, University of California, Los Angeles
Neel Chari, University of California, Los Angeles

Document Type

Article - post-print

Publication Date

2009

Abstract

We combined Gal4-UAS and the FLP recombinase–FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.

Original Publication Citation

Evans, C., Olson, J., Ngo, K. et al. G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila. Nat Methods 6, 603–605 (2009). https://doi.org/10.1038/nmeth.1356

Digital Commons @ LMU & LLS Citation

Evans, Cory J.; Olson, John M.; Ngo, Kathy T.; Kim, Eunha; Lee, Noemi E.; Kuoy, Edward; Patananan, Alexander N.; Sitz, Daniel; Tran, Phuong Thao; Do, Minh Tu; Yackle, Kevin; Cespedes, Albert; Hartenstein, Volker; Call, Gerald B.; Banerjee, Utpal; Beyder, Miriam; Bhatt, Kush V.; Bhoot, Chinmay; Bradshaw, Aaron W.; Brannigan, Tierney G.; Cao, Boyu; Cashell, Yancey Y.; Chai, Timothy; Chan, Alex W.; Chan, Carissa; Chang, Inho; Chang, Jonathan; Chang, Michael T.; Chang, Patrick W.; Chang, Stephen; and Chari, Neel, "G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila" (2009). Biology Faculty Works. 81.
https://digitalcommons.lmu.edu/bio_fac/81